Nickel Iminodiacetic acid NUPharose Fast Flow

Product Name:	Nickel Iminodiacetic acid NUPharose Fast Flow
Alternative Name:	Ni-IDA NUPharose FF
Catalog Number:	NRPB07

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Description

Matrix ：Cross-linked agarose, 6%

Ligand ：Iminodiacetic acid, ~30 μmol Ni²⁺/mL (capacity)

Particle size range： 45~165 μm

Particle size, d50^a ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥40 mg his-tagged protein/mL

Recommended operating flow velocity^c ：60~300 cm/h

Maximum flow velocity^d：1500 cm/h, 0.5 MPa

pH stability ：4~8.5 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)

Chemical stability ：Stable to common aqueous buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol.

Storage & Shipping ：20% ethanol, 2~8 ℃, shipping 2~30 ℃

Shelf life ：5 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^bDynamic binding capacity at 10% breakthrough for E. coli expressed recombinant Staphylococcus aureus protein A with a residence time of 6 mins; ^cThe flow rate can meet the use conditions of 2-6 mins residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.